FracFixR
Compositional Statistical Framework for RNA Fractionation Analysis
A compositional statistical framework for absolute proportion estimation between fractions in RNA sequencing data. 'FracFixR' addresses the fundamental challenge in fractionated RNA-seq experiments where library preparation and sequencing depth obscure the original proportions of RNA fractions. It reconstructs original fraction proportions using non-negative linear regression, estimates the "lost" unrecoverable fraction, corrects individual transcript frequencies, and performs differential proportion testing between conditions. Supports any RNA fractionation protocol including polysome profiling, sub-cellular localization, and RNA-protein complex isolation.
Versions across snapshots
| Version | Repository | File | Size |
|---|---|---|---|
1.0.0 |
rolling source/ R- | FracFixR_1.0.0.tar.gz |
135.7 KiB |
1.0.0 |
rolling linux/jammy R-4.5 | FracFixR_1.0.0.tar.gz |
171.2 KiB |
1.0.0 |
latest source/ R- | FracFixR_1.0.0.tar.gz |
135.7 KiB |
1.0.0 |
latest linux/jammy R-4.5 | FracFixR_1.0.0.tar.gz |
171.2 KiB |
1.0.0 |
2026-04-23 source/ R- | FracFixR_1.0.0.tar.gz |
135.7 KiB |
1.0.0 |
2026-04-09 windows/windows R-4.5 | FracFixR_1.0.0.zip |
173.0 KiB |
Dependencies (latest)
Imports
- future.apply (>= 1.8.1)
- nnls (>= 1.4)
- ggplot2 (>= 3.3.5)
- dplyr (>= 1.0.7)
- RColorBrewer (>= 1.1-2)
- tidyr (>= 1.1.3)
- matrixStats (>= 0.60.0)
- aod (>= 1.3.1)
- stats
- utils
- future
- grDevices